荧光光纤氧气测量技术具有高精确度、高可靠性、响应时间短、适用于气相和液相等优势，因此随着技术的问世，精确、高通量测量微小生物的呼吸和评估其能量代谢成为可能。高通量呼吸测量系统基于荧光光纤氧气测量技术，能够对斑马鱼的胚胎及幼鱼进行测量，测定其耗氧量，进而评估其代谢水平。系统在生物医学、实验生物学、污染生态学与环境毒理学、环境科学、气候变化研究等领域具有越来越重要的应用价值。

左：斑马鱼微型呼吸室；右：斑马鱼高通量呼吸代谢测量系统测量原理

  系统由内置荧光光纤氧气传感器的微型呼吸室、氧气测量主机及数据采集分析软件组成，可对96个通道的样品进行同步测量。

功能特点

• 氧气测量高精度、高可靠性、低功耗、低交叉敏感性、快速响应时间
• 轻松校准
• 非侵入性和非破坏性测量
• 紧凑设计，适用于温控培养箱和/或摇床

技术参数

1. 检测技术：光纤氧传感器技术。
2. 适用场景：原位检测，可在培养箱里或摇床上使用，便于温度控制。
3. 呼吸室：透明聚苯乙烯材质，支持预消毒处理，可重复使用。
4. 氧气测量主机：单个重670 g，162 x 102 x 32 mm
5. 主机内置温度传感器：0-50°C，分辨率012°C，精度±0.5°C
6. 主机内置压强传感器：300-1100mbar，分辨率11mbar，精度±6mbar
7. 最大采样频率：单通道激活时可达10-20次每秒
8. 氧气测量精度：±0.1% O2@1% O2或±0.05 mg/L@0.44 mg/L
9. 氧气测量分辨率：01% O2@1% O2或0.005 mg/L@0.44 mg/L
10. 电源：5VDC，USB供电
11. 响应时间＜30s
12. 通道数：96
13. 系统适配其他鱼类的胚胎及幼鱼
14. 可选配斑马鱼成鱼的静态及动态呼吸测量系统

左图：环境污染物6PPD和6PPD醌对斑马鱼幼鱼呼吸的影响(Varshney et al., 2022)；

右图：小丑鱼幼鱼在不同温度下的代谢率(Moore et al., 2023)

参考文献

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