时间:2020-02-17
作者:易科泰
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简介:
FL6000双调制叶绿素荧光测量仪
FL6000双调制叶绿素荧光仪是FL3500双调制叶绿素荧光仪的最新升级版,专门用于对蓝绿藻或绿藻等微藻,叶绿体或类囊体悬浮物进行光合作用深入机理研究的强大科研工具。仪器具备双通道测量控制,可控制测量样品的温度,并配备单翻转光(STF),内置多种可用户自行修改的测量程序,可进行目前国际上对于叶绿素荧光的各种深入机理研究。其核心结构是包含了一个悬浮液标准样品杯的光学测量头,内置3组LED光源和1个1MHz/16位 AD 转换的PIN二极管信号检测器。AD转换的增益和积分时间可以通过软件控制。检测器测量叶绿素荧光信号的时间分辨率可高达4 µs(快速版为1µs)。
应用领域:
典型样品:
功能特点:
技术参数:
1. 实验程序:Kautsky叶绿素荧光诱导效应测量;PAM(脉冲调制)荧光淬灭动力学测量;OJIP快速荧光动力学测量;QA–再氧化动力学;S状态转换;快速叶绿素荧光诱导
2. 荧光参数:
QA–再氧化动力学曲线和S-state test荧光衰减曲线(Li,2010)
3. 时间分辨率(采样频率):高灵敏度检测器,标准版时间分辨率为4µs,快速版为1µs
4. 最低检测极限:标准版100ng Chla/L,快速版1μg Chla/L
5. 控制单元:配备彩色触摸显示屏,可实时查看荧光曲线图
6. 测量室:
7. 定制测量室(选配):可分别定制测量光、饱和光闪和光化学光颜色(蓝色、青色、琥珀色等)以及检测波段(ChlA,ChlB)
8. 远红外光源(选配):用于测量F0',波长730nm
9. 氧气测量模块(选配):测量藻类的氧气释放
10. 温度控制(选配):TR 6000温度调节器,控温范围5–60℃,精确度0.1℃
11. 电磁搅拌(选配):用于样品混匀,防止样品沉淀,可手动调速或软件自动控制
12. 通讯接口:RS232串口/USB
13. FluorWin软件:定义或创建实验方案、光源控制设置、数据输出、分析处理和图表显示
典型应用:
1. 中科院水生生物所王强研究员使用FL3500叶绿素荧光仪(FL6000之前型号)和TL植物热释光系统证明亚硝酸盐胁迫首先影响Synechocystis sp. PCC 6803 PSII受体侧(Zhan X, et al, 2017)。这种光合作用深入机理的研究经常需要这两种仪器来配合完成。
2.中科院新疆生态与地理研究所潘响亮研究员及其课题组使用FL3500叶绿素荧光仪(FL6000之前型号)深入开展了环境中重金属、盐分、有毒化合物、除草剂、杀虫剂、抗生素等各种有害物质对藻类的毒理研究。通过FL3500独有的高分辨率OJIP快速荧光动力学测量、QA–再氧化动力学、S状态转换等叶绿素荧光测量程序,全面揭示了不同浓度与处理时间对藻类光合系统造成损伤的毒理机制及其生态影响。目前,潘响亮课题组已经使用FL3500(FL6000之前型号)在国际SCI期刊与国内核心期刊上发表了二十余篇高水平文章。
产地:捷克
参考文献:
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